Cerebro humano4/9/2023 Immediately the brain cuts were placed in dehydration in 100 % acetone, at 25 ° C, for 7 days the first acetone bath, and for another 3 more days, for the second acetone bath. Once the washing was finished, it was sectioned with a precision saw band machine, with stainless steel blade, obtaining 30 thin slices of 3 mm thickness. This brain was subsequently washed in running water for two weeks. The sample used consisted of a human brain, fixed and preserved with 10% formalin for 6 months. The aim of this communication was to analyze and establish the shrinkage of brain sections preserved by a classic protocol of sheet plastination with polyester resin Biodur® P40 in 3 mm thick human brain slices ( Guerrero et al., 2019). In relation to this disadvantage of the technique, it is necessary to establish statistically the shrinkage percentages. But in relation to sheet plastination with epoxy resin, polyester technique causes marked shrinkage of the tissues. Likewise, this technique can be applied to any body region (Ottone et al., 2014 Ottone et al., 2018a,b Prieto et al., 2019). In particular, sheet plastination with polyester resin was initially created for the preservation of brain slices, for the identification of gray and white matters (von Hagens, 1987). Cell Biology, Bethesda, Maryland.Plastination is an anatomical cadaveric conservation technique created in 1977 by Gunther von Hagens, in Heidelberg, Germany ( Ottone 2013, 2018), and which replaces biological and/or fixation fluids with an intermediate solvent (acetone), to then impregnate the samples with different polymers, depending on the technique of plastination chosen, to finally carry out the polymerization of the components incorporated into the samples, to obtain dry and totally durable biological samples (Ottone et al., 2015 Ottone et al., 2016 Vargas et al., 2019). Swanson APPENDIX Tables of Data on the Nucleus (Adapted by kind permission of Federation of American Societies for Experimental Biology from Biological Poulos, Ranjan Batra, Konstantinos Charizanis, and Maurice S. Orr Developments in RNA Splicing and Disease Michael G. Worman, Cecilia Östlund, and Yuexia Wang Higher-order Genome Organization in Human Disease Tom Misteli Nuclear Ataxias Harry T. ![]() Singer, and David Grünwald NUCLEAR ARCHITECTURE IN DIFFERENTIATION AND DISEASE Diseases of the Nuclear Envelope Howard J. Cristina Cardoso DNA Damage Response Giuseppina Giglia-Mari, Angelika Zotter, and Wim Vermeulen RNA Processing and Export Sami Hocine, Robert H. Berciano, and Miguel Lafarga Biogenesis of Nuclear Bodies Miroslav Dundr and Tom Misteli FUNCTIONAL ORGANIZATION Organization of Transcription Lyubomira Chakalova and Peter Fraser Organization of DNA Replication Vadim O. Lamond Nuclear Stress Bodies Giuseppe Biamonti and Claire Vourc’h Orphan Nuclear Bodies Maria Carmo-Fonseca, Maria T. ![]() Gall PML Nuclear Bodies Valérie Lallemand-Breitenbach and Hugues de Thé The Perinucleolar Compartment Callie Pollock and Sui Huang Paraspeckles Archa H. Lamond The Cajal Body and Histone Locus Body Zehra Nizami, Svetlana Deryusheva, and Joseph G. Gasser Nuclear Functions of Actin Neus Visa and Piergiorgio Percipalle NUCLEAR BODIES The Nucleolus Thoru Pederson Nuclear Speckles David L. ![]() Chow and Edith Heard The Budding Yeast Nucleus Angela Taddei, Heiko Schober, and Susan M. Ghosh Nuclear Organization and Dosage Compensation Jennifer C. Rout CHROMOSOMES & CHROMATIN Chromosome Territories Thomas Cremer and Marion Cremer Gene Positioning Carmelo Ferrai, Inês Jesus de Castro, Liron Lavitas, Mita Chotalia, and Ana Pombo Chromatin Higher-order Structure and Dynamics Christopher L. Wilson and Roland Foisner The Nuclear Pore Complex and Nuclear Transport Susan R. Goldman Lamin-binding Proteins Katherine L. Adam, Pekka Taimen, Takeshi Shimi, and Robert D. Hetzer Nuclear Lamins Thomas Dechat, Stephen A. OVERVIEW The Nucleus Introduced Thoru Pederson THE NUCLEAR PERIPHERY The Nuclear Envelope Martin W.
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